qualityScores - Online in the Cloud

PROGRAM:

NAME

qualityScores - a component of subread suite

USAGE

qualityScores [options] -i <input_file> -o <output_file>

Required arguments:

-i <string>
Name of input file including read data. The default format is Fastq.

-o <string>
Name of output file that is a text file including Phred scores for each read base.

Optional arguments:

--gzFASTQinput Input file is in gzipped Fastq format.

--BAMinput
Input file is in BAM format.

--SAMinput
Input file is in SAM format.

--first-end
Use only first reads in paired-end data. Only applicable for paired-end BAM/SAM
input.

--second-end
Use only second reads in paired-end data. Only applicable for paired-end BAM/SAM
input.

--counted-reads <int> Total number of reads to be extracted from the input

file. 10,000 by default.

--phred-offset <33|64> refer to subread aligner.

Use qualityScores online using onworks.net services