velvetg_63 - Online in the Cloud

PROGRAM:

NAME

velvetg_63 - de Bruijn graph construction, error removal and repeat resolution
(MAXKMERLENGTH = 63)

DESCRIPTION

Usage: ./velvetg directory [options]

directory
: working directory name

Standard options:
-cov_cutoff <floating-point|auto>
: removal of low coverage nodes AFTER tour bus or allow the system to infer it

(default: no removal)

-ins_length <integer>
: expected distance between two paired end reads (default: no read pairing)

-read_trkg <yes|no>
: tracking of short read positions in assembly (default: no tracking)

-min_contig_lgth <integer>
: minimum contig length exported to contigs.fa file (default: hash length * 2)

-amos_file <yes|no>
: export assembly to AMOS file (default: no export)

-exp_cov <floating point|auto>
: expected coverage of unique regions or allow the system to infer it

(default: no long or paired-end read resolution)

-long_cov_cutoff <floating-point>: removal of nodes with low long-read coverage AFTER tour
bus

(default: no removal)

Advanced options:
-ins_length* <integer>
: expected distance between two paired-end reads in the respective short-read
dataset (default: no read pairing)

-ins_length_long <integer>
: expected distance between two long paired-end reads (default: no read pairing)

-ins_length*_sd <integer>
: est. standard deviation of respective dataset (default: 10% of corresponding
length)

[replace '*' by nothing, '2' or '_long' as necessary]

-scaffolding <yes|no>
: scaffolding of contigs used paired end information (default: on)

-max_branch_length <integer>
: maximum length in base pair of bubble (default: 100)

-max_divergence <floating-point>: maximum divergence rate between two branches in a bubble
(default: 0.2)

-max_gap_count <integer>
: maximum number of gaps allowed in the alignment of the two branches of a bubble
(default: 3)

-min_pair_count <integer>
: minimum number of paired end connections to justify the scaffolding of two long
contigs (default: 5)

-max_coverage <floating point>
: removal of high coverage nodes AFTER tour bus (default: no removal)

-coverage_mask <int>
: minimum coverage required for confident regions of contigs (default: 1)

-long_mult_cutoff <int>
: minimum number of long reads required to merge contigs (default: 2)

-unused_reads <yes|no>
: export unused reads in UnusedReads.fa file (default: no)

-alignments <yes|no>
: export a summary of contig alignment to the reference sequences (default: no)

-exportFiltered <yes|no>
: export the long nodes which were eliminated by the coverage filters (default: no)

-clean <yes|no>
: remove all the intermediary files which are useless for recalculation (default :
no)

-very_clean <yes|no>
: remove all the intermediary files (no recalculation possible) (default: no)

-paired_exp_fraction <double>
: remove all the paired end connections which less than the specified fraction of
the expected count (default: 0.1)

-shortMatePaired* <yes|no>
: for mate-pair libraries, indicate that the library might be contaminated with
paired-end reads (default no)

-conserveLong <yes|no>
: preserve sequences with long reads in them (default no)

Output:
directory/contigs.fa
: fasta file of contigs longer than twice hash length

directory/stats.txt
: stats file (tab-spaced) useful for determining appropriate coverage cutoff

directory/LastGraph
: special formatted file with all the information on the final graph

directory/velvet_asm.afg
: (if requested) AMOS compatible assembly file

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