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easy-dhpsf to run in Linux online download for Linux

Free download easy-dhpsf to run in Linux online Linux app to run online in Ubuntu online, Fedora online or Debian online

This is the Linux app named easy-dhpsf to run in Linux online whose latest release can be downloaded as easy-dhpsf_v2_1.zip. It can be run online in the free hosting provider OnWorks for workstations.

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easy-dhpsf to run in Linux online


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DESCRIPTION

This package of MATLAB routines with accompanying GUI allows scientific users to measure the 3D location of single molecules when using the double-helix point spread function (DH-PSF) widefield microscope. Version 2 also includes code that facilitates combining localization data from two spectral channels using a locally-weighted quadratic 3D registration function

Features
- Double-Gaussian estimator is calibrated via an axial scan of bright immobile fluorescent emitters (e.g. beads)
- DH localization is performed through template matching and subsequent double Gaussian fitting
- Tiff stacks of SM images are analyzed using template matching followed by double-Gaussian fitting to extract estimates of the molecule positions
- Two spectral channels are registered by generating a locally-weighted quadratic mapping function from control point pairs

Audience

Science/Research



Programming Language

MATLAB



This is an application that can also be fetched from https://sourceforge.net/projects/easy-dhpsf/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.


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