This is the Windows app named easy-dhpsf whose latest release can be downloaded as easy-dhpsf_v2_1.zip. It can be run online in the free hosting provider OnWorks for workstations.
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easy-dhpsf
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DESCRIPTION
This package of MATLAB routines with accompanying GUI allows scientific users to measure the 3D location of single molecules when using the double-helix point spread function (DH-PSF) widefield microscope. Version 2 also includes code that facilitates combining localization data from two spectral channels using a locally-weighted quadratic 3D registration function
Features
- Double-Gaussian estimator is calibrated via an axial scan of bright immobile fluorescent emitters (e.g. beads)
- DH localization is performed through template matching and subsequent double Gaussian fitting
- Tiff stacks of SM images are analyzed using template matching followed by double-Gaussian fitting to extract estimates of the molecule positions
- Two spectral channels are registered by generating a locally-weighted quadratic mapping function from control point pairs
Audience
Science/Research
Programming Language
MATLAB
Categories
This is an application that can also be fetched from https://sourceforge.net/projects/easy-dhpsf/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.
