This is the command kmc_tools-kmers_subtract that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
kmc_tools-kmers_subtract - calculate subtraction of two KMC databases
SYNOPSIS
kmc_tools kmers_subtract <input1 [input1_params]> <input2 [input2_params]> <output
[output_params]>
DESCRIPTION
The output database will contains only k-mers that are present in the first input set but
absent in the second one. The counter value is equal to the value from the first input
set.
OPTIONS
For each input there are additional parameters:
-ci<value>
exclude k-mers occurring less than <value> times
-cx<value>
exclude k-mers occurring more of than <value> times
For output there are additional parameters:
-ci<value>
exclude k-mers occurring less than <value> times
-cx<value>
exclude k-mers occurring more of than <value> times
-cs<value>
maximal value of a counter
EXAMPLE
kmc -k28 file1.fastq kmers1 tmp
kmc -k28 file2.fastq kmers2 tmp
kmc_tools kmers_subtract kmers1 kmers2 kmers1_kmers2_subtract -cs200
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