This is the command micro_razers that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
micro_razers
SYNOPSIS
micro_razers [OPTIONS] <GENOME FILE> <READS FILE>
DESCRIPTION
MicroRazerS uses a prefix-based mapping strategy to map small RNA reads possibly
containing 3' adapter sequence.
(c) Copyright 2009 by Anne-Katrin Emde.
-h, --help
Displays this help message.
--version
Display version information
Main Options::
-o, --output FILE
Change output filename. Default: <READS FILE>.result.
-rr, --recognition-rate NUM
set the percent recognition rate In range [80..100]. Default: 100.
-sL, --seed-length NUM
seed length In range [10..inf]. Default: 16.
-sE, --seed-error
allow for one error in the seed
-f, --forward
map reads only to forward strands.
-r, --reverse
map reads only to reverse strands.
-mN, --match-N
'N' matches with all other characters
-m, --max-hits NUM
output only NUM of the best hits In range [1..inf]. Default: 100.
-pa, --purge-ambiguous
purge reads with more than max-hits best matches
-lm, --low-memory
decrease memory usage at the expense of runtime
-v, --verbose
verbose mode
-vv, --vverbose
very verbose mode
Output Format Options::
-of, --output-format NUM
Set output format. 0 = MicroRazerS format, 1 = SAM. In range [0..1].
-a, --alignment
dump the alignment for each match
-gn, --genome-naming NUM
Select how genomes are named. 0 = use Fasta id, 1 = enumerate beginning with 1. In
range [0..1]. Default: 0.
-rn, --read-naming NUM
Select how reads are named. 0 = use Fasta id, 1 = enumerate beginning with 1. In
range [0..1]. Default: 0.
-so, --sort-order NUM
Select how matches are sorted. 0 = read number, 1 = genome position. In range
[0..1]. Default: 0.
-pf, --position-format NUM
Select begin/end position numbering (see Coordinate section below). 0 = gap space,
1 = position space. In range [0..1]. Default: 0.
VERSION
micro_razers version: 1.0.1 Last update Jul 2009
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