This is the command msbare that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
msbar - Mutate a sequence
SYNOPSIS
msbar -sequence seqall -othersequence seqall -count integer -point list -block list
-codon list -inframe boolean -minimum integer -maximum integer -outseq seqoutall
msbar -help
DESCRIPTION
msbar is a command line program from EMBOSS (“the European Molecular Biology Open Software
Suite”). It is part of the "Nucleic:Mutation,Protein:Mutation" command group(s).
OPTIONS
Input section
-sequence seqall
-othersequence seqall
If you require that the resulting mutated sequence should not match a set of other
sequences, then you can specify that set of sequences here. For example, if you
require that the mutated sequence should not be the same as the input sequence, enter
the input sequence here. If you want the result to be different to previous results of
this program, specify the previous result sequences here. The program will check that
the result does not match the sequences specified here before writing it out. If a
match is found, then the mutation is started again with a fresh copy of the input
sequence. If, after 10 such retries, there is still a match to the set of sequence
given here, then the matching mutated sequence is written with a warning message.
Default value: asis:N
Required section
-count integer
Default value: 1
-point list
-block list
-codon list
Types of codon mutations to perform. These are only done if the sequence is nucleic.
Additional section
-inframe boolean
Default value: N
Advanced section
-minimum integer
Default value: 1
-maximum integer
Default value: 10
Output section
-outseq seqoutall
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