pbalign - Online in the Cloud

PROGRAM:

NAME

pbalign - Mapping PacBio sequences to references

DESCRIPTION

usage: pbalign [-h] [--verbose] [--version] [--profile] [--debug]

[--regionTable REGIONTABLE] [--configFile CONFIGFILE] [--pulseFile PULSEFILE]
[--algorithm {blasr,bowtie,gmap}] [--maxHits MAXHITS] [--minAnchorSize
MINANCHORSIZE] [--useccs {useccs,useccsall,useccsdenovo}] [--noSplitSubreads]
[--concordant] [--nproc NPROC] [--algorithmOptions ALGORITHMOPTIONS]
[--maxDivergence MAXDIVERGENCE] [--minAccuracy MINACCURACY] [--minLength MINLENGTH]
[--scoreFunction {alignerscore,editdist,blasrscore}] [--scoreCutoff SCORECUTOFF]
[--hitPolicy {randombest,allbest,random,all,leftmost}] [--filterAdapterOnly]
[--forQuiver] [--loadQVs] [--byread] [--metrics METRICS] [--seed SEED] [--tmpDir
TMPDIR] inputFileName referencePath outputFileName

Mapping PacBio sequences to references using an algorithm selected from a selection of
supported command-line alignment algorithms. Input can be a fasta, pls.h5, bas.h5 or
ccs.h5 file or a fofn (file of file names). Output is in either cmp.h5 or sam format.

positional arguments:
inputFileName
The input file can be a fasta, plx.h5, bax.h5, ccs.h5 file or a fofn.

referencePath
Either a reference fasta file or a reference repository.

outputFileName
The output cmp.h5 or sam file.

optional arguments:
-h, --help
show this help message and exit

--verbose, -v
Set the verbosity level

--version
show program's version number and exit

--profile
Print runtime profile at exit

--debug
Catch exceptions in debugger (requires ipdb)

--regionTable REGIONTABLE
Specify a region table for filtering reads.

--configFile CONFIGFILE
Specify a set of user-defined argument values.

--pulseFile PULSEFILE
When input reads are in fasta format and output is a cmp.h5 this option can specify
pls.h5 or bas.h5 or FOFN files from which pulse metrics can be loaded for Quiver.

--algorithm {blasr,bowtie,gmap}
Select an aligorithm from ('blasr', 'bowtie', 'gmap'). Default algorithm is blasr.

--maxHits MAXHITS
The maximum number of matches of each read to the reference sequence that will be
evaluated. Default value is 10.

--minAnchorSize MINANCHORSIZE
The minimum anchor size defines the length of the read that must match against the
reference sequence. Default value is 12.

--useccs {useccs,useccsall,useccsdenovo}
Map the ccsSequence to the genome first, then align subreads to the interval that
the CCS reads mapped to.

useccs: only maps subreads that span the length of
the template.

useccsall: maps all subreads.
useccsdenovo: maps ccs only.

--noSplitSubreads
Do not split reads into subreads even if subread regions are available. Default
value is False.

--concordant
Map subreads of a ZMW to the same genomic location.

--nproc NPROC
Number of threads. Default value is 8.

--algorithmOptions ALGORITHMOPTIONS
Pass alignment options through.

--maxDivergence MAXDIVERGENCE
The maximum allowed percentage divergence of a read from the reference sequence.
Default value is 30.

--minAccuracy MINACCURACY
The minimum percentage accuracy of alignments that will be evaluated. Default value
is 70.

--minLength MINLENGTH
The minimum aligned read length of alignments that will be evaluated. Default value
is 50.

--scoreFunction {alignerscore,editdist,blasrscore}
Specify a score function for evaluating alignments.

alignerscore : aligner's score in the SAM tag 'as'.
editdist : edit distance between read and reference. blasrscore : blasr's
default score function.

Default value is alignerscore.

--scoreCutoff SCORECUTOFF
The worst score to output an alignment.

--hitPolicy {randombest,allbest,random,all,leftmost}
Specify a policy for how to treat multiple hit

random : selects a random hit.

all : selects all hits.

allbest
: selects all the best score hits.

randombest: selects a random hit from all best score hits.
leftmost : selects a hit which has the best score and the

smallest mapping coordinate in any reference.
Default value is randombest.

--filterAdapterOnly
If specified, do not report adapter-only hits using annotations with the reference
entry.

--forQuiver
The output cmp.h5 file which will be sorted, loaded with pulse QV information, and
repacked, so that it can be consumed by quiver directly. This requires the input
file to be in PacBio bas/pls.h5 format, and --useccs must be None. Default value is
False.

--loadQVs
Similar to --forQuiver, the only difference is that --useccs can be specified.
Default value is False.

--byread
Load pulse information using -byread option instead of -bymetric. Only works when
--forQuiver or --loadQVs are set. Default value is False.

--metrics METRICS
Load the specified (comma-delimited list of) metrics instead of the default metrics
required by quiver. This option only works when --forQuiver or --loadQVs are set.
Default: DeletionQV,DeletionTag,InsertionQV,MergeQV,SubstitutionQV

--seed SEED
Initialize the random number generator with a none-zero integer. Zero means that
current system time is used. Default value is 1.

--tmpDir TMPDIR
Specify a directory for saving temporary files. Default is /scratch.

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