This is the command primae that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
prima - Selects primers for PCR and DNA amplification.
SYNOPSIS
prima -sequence sequence -targetrange toggle -targetstart integer -targetend integer
-mintmprimer float -maxtmprimer float -minplen integer -maxplen integer
-minpgccont float -maxpgccont float [-primer toggle] -minprimerlen integer
-maxprimerlen integer -minpmgccont float -maxpmgccont float -overlap integer
-saltconc float -dnaconc float -list boolean -outfile outfile
prima -help
DESCRIPTION
prima is a command line program from EMBOSS (“the European Molecular Biology Open Software
Suite”). It is part of the "Nucleic:Primers" command group(s).
OPTIONS
Input section
-sequence sequence
Required section
Target options
-targetrange toggle
Default value: N
-targetstart integer
Default value: $(sequence.begin)
-targetend integer
Default value: $(sequence.length)
-mintmprimer float
Default value: 53
-maxtmprimer float
Default value: 58
Additional section
Product options
-minplen integer
Default value: 100
-maxplen integer
Default value: 300
-minpgccont float
Default value: .40
-maxpgccont float
Default value: .55
Primer options
-primer toggle
Specify primer properties Default value: Y
-minprimerlen integer
Default value: 18
-maxprimerlen integer
Default value: 22
-minpmgccont float
Default value: .40
-maxpmgccont float
Default value: .55
Advanced section
-overlap integer
Default value: 50
-saltconc float
Default value: 50
-dnaconc float
Default value: 50
-list boolean
Default value: N
Output section
-outfile outfile
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