This is the command proftmb that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
proftmb - per-residue prediction of bacterial transmembrane beta barrels
SYNOPSIS
proftmb [options]
DESCRIPTION
proftmb predicts bacterial transmembrane beta barrels from sequence using profile fed
Hidden Markov Models (HMM).
Output format
Self-annotating. Letters of prediction: 'i' - inside cytoplasm, 'O' - outside cytoplasm,
'U' - up (on chain moving out of cytoplasm), 'D' - down (on chain moving into cytoplasm).
References
Bigelow, H. and Rost, B. (2006). PROFtmb: a web server for predicting bacterial
transmembrane beta barrel proteins. Nucleic Acids Res, 34(Web Server issue), W186-8.
To invoke the menu, type:
proftmb --menu
The options are:
-d, --directory-root <STRING>
root path where files (options -s,-r,-l,-a,-e,-t,-u,-z,-n) reside
-a, --reduction-state-decode <STRING>
state reduction for decoding
-b, --reduction-state-report <STRING>
state reduction for reporting
-m, --membrane-strand-states <STRING>
list of membrane strand states
-z, --z-curve-file
<STRING> file containing means and sd's at integral length values
-x, --z-calibration-curve <STRING>
file mapping coverage and accuracy values to z-scores
-n, --null-frequency <STRING>
background frequency file
-c, --minimum-score-cutoff <DOUBLE>
minimum z-score for per-residue prediction
-o, --outfile-prefix <STRING>
output file prefix for the three files generated: PREFIX_dat.txt,
PREFIX_proftmb_pretty.txt PREFIX_proftmb_tabular.txt
-s, --static-model-data <STRING>
data representing the model architecture
-t, --trained-params <STRING>
params representing the encoded training data
-q, --test-blastQ-file-or-dir <STRING>
psiblast profile (-Q) or directory (full pathname or relative to current directory)
with many profiles
-w, --single-sequence-name <STRING>
if -q option points to a single file, this is the name
-v, --list-blastQ-files <STRING>
list of psiblast files to process in directory (leave blank to process all files)
--outfile-pretty <STRING>
pretty output file (overrides automatic name PREFIX_proftmb_pretty.txt)
--outfile-tab
<STRING> tabulated output file (overrides automatic name
PREFIX_proftmb_tabular.txt)
--outfile-dat
<STRING> data output file (overrides automatic name PREFIX_dat.txt)
--version
<BOOL> output version information and exit
--quiet
<BOOL> be quiet
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