readseq - Online in the Cloud

PROGRAM:

NAME

readseq - Reads and writes nucleic/protein sequences in various formats

SYNOPSIS

readseq [-options] in.seq > out.seq

DESCRIPTION

This manual page documents briefly the readseq command. This manual page was written for
the Debian GNU/Linux distribution because the original program does not have a manual
page. Instead, it has documentation in text form, see below.

readseq reads and writes biosequences (nucleic/protein) in various formats. Data files
may have multiple sequences. readseq is particularly useful as it automatically detects
many sequence formats, and interconverts among them.

FORMATS

Formats which readseq currently understands:

* IG/Stanford, used by Intelligenetics and others
* GenBank/GB, genbank flatfile format
* NBRF format
* EMBL, EMBL flatfile format
* GCG, single sequence format of GCG software
* DNAStrider, for common Mac program
* Fitch format, limited use
* Pearson/Fasta, a common format used by Fasta programs and others
* Zuker format, limited use. Input only.
* Olsen, format printed by Olsen VMS sequence editor. Input only.
* Phylip3.2, sequential format for Phylip programs
* Phylip, interleaved format for Phylip programs (v3.3, v3.4)
* Plain/Raw, sequence data only (no name, document, numbering)
+ MSF multi sequence format used by GCG software
+ PAUP's multiple sequence (NEXUS) format
+ PIR/CODATA format used by PIR
+ ASN.1 format used by NCBI
+ Pretty print with various options for nice looking output. Output only.
+ LinAll format, limited use (LinAll and ConStruct programs)
+ Vienna format used by ViennaRNA programs

See the included "Formats" file for detail on file formats.

OPTIONS

-help Show summary of options.

-a[ll] Select All sequences

-c[aselower]
Change to lower case

-C[ASEUPPER]
Change to UPPER CASE

-degap[=-]
Remove gap symbols

-i[tem=2,3,4]
Select Item number(s) from several

-l[ist]
List sequences only

-o[utput=]out.seq
Redirect Output

-p[ipe]
Pipe (command line, <stdin, >stdout)

-r[everse]
Change to Reverse-complement

-v[erbose]
Verbose progress

-f[ormat=]# Format number for output, or
-f[ormat=]Name Format name for output:
1. IG/Stanford 11. Phylip3.2
2. GenBank/GB 12. Phylip
3. NBRF 13. Plain/Raw
4. EMBL 14. PIR/CODATA
5. GCG 15. MSF
6. DNAStrider 16. ASN.1
7. Fitch 17. PAUP/NEXUS
8. Pearson/Fasta 18. Pretty (out-only)
9. Zuker (in-only) 19. LinAll
10. Olsen (in-only) 20. Vienna

Pretty format options:

-wid[th]=#
Sequence line width

-tab=# Left indent

-col[space]=#
Column space within sequence line on output

-gap[count]
Count gap chars in sequence numbers

-nameleft, -nameright[=#]
Name on left/right side [=max width]

-nametop
Name at top/bottom

-numleft, -numright
Seq index on left/right side

-numtop, -numbot
Index on top/bottom

-match[=.]
Use match base for 2..n species

-inter[line=#]
Blank line(s) between sequence blocks

EXAMPLES

readseq
-- for interactive use

readseq my.1st.seq my.2nd.seq -all -format=genbank -output=my.gb
-- convert all of two input files to one genbank format output file

readseq my.seq -all -form=pretty -nameleft=3 -numleft -numright -numtop -match
-- output to standard output a file in a pretty format

readseq my.seq -item=9,8,3,2 -degap -CASE -rev -f=msf -out=my.rev
-- select 4 items from input, degap, reverse, and uppercase them

cat *.seq | readseq -pipe -all -format=asn > bunch-of.asn
-- pipe a bunch of data thru readseq, converting all to asn

Use readseq online using onworks.net services