This is the command seqaligne that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
seqalign - Extend alignments (DAF file) with sequences (DHF file).
SYNOPSIS
seqalign -mode list -dhfinpath dirlist -dafinpath dirlist -dhfindir directory -amode list
[-forcetype boolean] -dafoutdir outdir -logfile outfile
seqalign -help
DESCRIPTION
seqalign is a command line program from EMBOSS (“the European Molecular Biology Open
Software Suite”). It is part of the "Protein:3D Structure" command group(s).
OPTIONS
Input section
-mode list
This option specifies the mode of SEQALIGN operation. SEQALIGN takes as input a
directory of either i. single sequences, ii. set of sequences (unaligned or aligned,
but typically aligned sequences within a domain alignment file)). The user has to
specify which. Default value: 1
-dhfinpath dirlist
This option specifies the location of sequences, e.g. DHF files (domain hits files)
(input). SEQALIGN takes as input a database of either i. single sequences, ii. sets of
unaligned sequences or iii. sets of aligned sequences, e.g. a domain alignment file. A
'domain alignment file' contains a sequence alignment of domains belonging to the same
SCOP or CATH family. The file is in clustal format annotated with domain family
classification information. The files generated by using SCOPALIGN will contain a
structure-based sequence alignment of domains of known structure only. Such alignments
can be extended with sequence relatives (of unknown structure) by using SEQALIGN.
Default value: ./
-dafinpath dirlist
This option specifies the location of sequences, e.g. DAF files (domain alignment
files) (input). SEQALIGN takes as input a database of either i. single sequences, ii.
sets of unaligned sequences or iii. sets of aligned sequences, e.g. a domain alignment
file. A 'domain alignment file' contains a sequence alignment of domains belonging to
the same SCOP or CATH family. The file is in clustal format annotated with domain
family classification information. The files generated by using SCOPALIGN will contain
a structure-based sequence alignment of domains of known structure only. Such
alignments can be extended with sequence relatives (of unknown structure) by using
SEQALIGN. Default value: ./
-dhfindir directory
This option specifies the location of DHF files (domain hits files) (input). A 'domain
hits file' contains database hits (sequences) with domain classification information,
in the DHF format (FASTA or EMBL-like). The hits are relatives to a SCOP or CATH
family and are found from a search of a sequence database. Files containing hits
retrieved by PSIBLAST are generated by using SEQSEARCH. Default value: ./
Required section
-amode list
This option specifies which alignment algorithm to use. Default value: 1
Additional section
-forcetype boolean
This option specifies whether to force minimal domain classification data to be
written to the output file in cases where singlet sequences were given as input file
and no classification data was available Default value: N
Output section
-dafoutdir outdir
This option specifies the location of DAF files (domain alignment files) (output). A
'domain alignment file' contains a sequence alignment of domains belonging to the same
SCOP or CATH family. The file is in clustal format annotated with domain family
classification information. The files generated by using SCOPALIGN will contain a
structure-based sequence alignment of domains of known structure only. Such alignments
can be extended with sequence relatives (of unknown structure) by using SEQALIGN.
Default value: ./
-logfile outfile
This option specifies the name of log file for the build. The log file contains
messages about any errors arising while SEQALIGN ran. Default value: seqalign.log
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