This is the command STAR that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
STAR - ultrafast universal RNA-seq aligner
DESCRIPTION
Spliced Transcripts Alignment to a Reference (STAR) software based on a previously
undescribed RNA-seq alignment algorithm that uses sequential maximum mappable seed search
in uncompressed suffix arrays followed by seed clustering and stitching procedure. STAR
outperforms other aligners by a factor of >50 in mapping speed, aligning to the human
genome 550 million 2 × 76 bp paired-end reads per hour on a modest 12-core server, while
at the same time improving alignment sensitivity and precision. In addition to unbiased de
novo detection of canonical junctions, STAR can discover non-canonical splices and
chimeric (fusion) transcripts, and is also capable of mapping full-length RNA sequences.
Using Roche 454 sequencing of reverse transcription polymerase chain reaction amplicons,
the authors experimentally validated 1960 novel intergenic splice junctions with an 80-90%
success rate, corroborating the high precision of the STAR mapping strategy.
SYNOPSIS
STAR --option1-name option1-value(s)--option2-name option2-value(s) ...
Use STAR online using onworks.net services
