EnglishFrenchSpanish

OnWorks favicon

sam-stats - Online in the Cloud

Run sam-stats in OnWorks free hosting provider over Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator

This is the command sam-stats that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator

PROGRAM:

NAME


sam-stats - ea-utils: produce digested statistics

SYNOPSIS


sam-stats [options] [file1] [file2...filen]

DESCRIPTION


Version: 1.38.681

Produces lots of easily digested statistics for the files listed

Options (default in parens):

-D Keep track of multiple alignments -O PREFIX Output prefix enabling
extended output (see below) -R FIL Coverage/RNA output (coverage, 3' bias, etc,
implies -A) -A Report all chr sigs, even if there are more than 1000 -b INT
Number of reads to sample for per-base stats (1M) -S INT Size of ascii-signature
(30) -x FIL File extension for handling multiple files (stats) -M Only
overwrite if newer (requires -x, or multiple files) -B Input is bam, don't
bother looking at magic -z Don't fail when zero entries in sam

OUTPUT:

If one file is specified, then the output is to standard out. If multiple files are
specified, or if the -x option is supplied, the output file is <filename>.<ext>. Default
extension is 'stats'.

Complete Stats:

<STATS>
: mean, max, stdev, median, Q1 (25 percentile), Q3

reads : # of entries in the sam file, might not be # reads

phred : phred scale used

bsize : # reads used for qual stats

mapped reads
: number of aligned reads (unique probe id sequences)

mapped bases
: total of the lengths of the aligned reads

forward
: number of forward-aligned reads

reverse
: number of reverse-aligned reads

snp rate
: mismatched bases / total bases (snv rate)

ins rate
: insert bases / total bases

del rate
: deleted bases / total bases

pct mismatch
: percent of reads that have mismatches

pct align
: percent of reads that aligned

len <STATS>
: read length stats, ignored if fixed-length

mapq <STATS>
: stats for mapping qualities

insert <STATS>
: stats for insert sizes

%<CHR> : percentage of mapped bases per chr, followed by a signature

Subsampled stats (1M reads max):
base qual <STATS> : stats for base qualities %A,%T,%C,%G : base percentages

Meaning of the per-chromosome signature:
A ascii-histogram of mapped reads by chromosome position. It is only output if the
original SAM/BAM has a header. The values are the log2 of the # of mapped reads at
each position + ascii '0'.

Extended output mode produces a set of files:
.stats : primary output

.fastx : fastx-toolkit compatible output

.rcov : per-reference counts & coverage

.xdist : mismatch distribution

.ldist : length distribution (if applicable)

.mqdist
: mapping quality distribution

Use sam-stats online using onworks.net services


Free Servers & Workstations

Download Windows & Linux apps

  • 1
    strace
    strace
    The strace project has been moved to
    https://strace.io. strace is a
    diagnostic, debugging and instructional
    userspace tracer for Linux. It is used
    to monitor a...
    Download strace
  • 2
    gMKVExtractGUI
    gMKVExtractGUI
    A GUI for mkvextract utility (part of
    MKVToolNix) which incorporates most (if
    not all) functionality of mkvextract and
    mkvinfo utilities. Written in C#NET 4.0,...
    Download gMKVExtractGUI
  • 3
    JasperReports Library
    JasperReports Library
    JasperReports Library is the
    world's most popular open source
    business intelligence and reporting
    engine. It is entirely written in Java
    and it is able to ...
    Download JasperReports Library
  • 4
    Frappe Books
    Frappe Books
    Frappe Books is a free and open source
    desktop book-keeping software that's
    simple and well-designed to be used by
    small businesses and freelancers. It'...
    Download Frappe Books
  • 5
    Numerical Python
    Numerical Python
    NEWS: NumPy 1.11.2 is the last release
    that will be made on sourceforge. Wheels
    for Windows, Mac, and Linux as well as
    archived source distributions can be fou...
    Download Numerical Python
  • 6
    CMU Sphinx
    CMU Sphinx
    CMUSphinx is a speaker-independent large
    vocabulary continuous speech recognizer
    released under BSD style license. It is
    also a collection of open source tools ...
    Download CMU Sphinx
  • More »

Linux commands

crm
crm
Use crm online using onworks.net
services. ...
Run crm
  • 4
    crmgr
    crmgr
    crmgr - administration utility for QDBM
    Curia ...
    Run crmgr
  • 5
    gappletviewer
    gappletviewer
    gappletviewer - Load and runs an applet
    ...
    Run gappletviewer
  • 6
    gaps
    gaps
    mummer - package for sequence alignment
    of multiple genomes ...
    Run gaps
  • s-processed="true">
    g15stats
    g15stats - A CPU/Memory/Swap usage
    meter for G15Daemon DESCRIPTION: The
    packages provides the following usage
    meter for LCD on some Logitech
    keyboards, usind g...
    Run g15stats
  • More »
  • Ad