This is the Linux app named ArtificialFastqGenerator whose latest release can be downloaded as ArtificialFastqGenerator_19_05_2015.zip. It can be run online in the free hosting provider OnWorks for workstations.
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ArtificialFastqGenerator
DESCRIPTION:
ArtificialFastqGenerator takes the reference genome (in FASTA format) as input and outputs artificial FASTQ files in the Sanger format. It can accept Phred base quality scores from existing FASTQ files, and use them to simulate sequencing errors. Since the artificial FASTQs are derived from the reference genome, the reference genome provides a gold-standard for calling variants (Single Nucleotide Polymorphisms (SNPs) and insertions and deletions (indels)). This enables evaluation of a Next Generation Sequencing (NGS) analysis pipeline which aligns reads to the reference genome and then calls the variants.Audience
Healthcare Industry, Science/Research, Advanced End Users
Programming Language
Java
This is an application that can also be fetched from https://sourceforge.net/projects/artfastqgen/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.