This is the Linux app named Autoprime whose latest release can be downloaded as autoprime.2.1.tar.gz. It can be run online in the free hosting provider OnWorks for workstations.
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SCREENSHOTS
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Autoprime
DESCRIPTION
AutoPrime allows to rapidly design primers for real-time PCR measurement of eukaryotic expression.
By connecting to the EnsEMBL database gene structure information is used to find primers spanning exon borders. This way only expressed sequences are captured. Additionally mispriming on repetitive regions can be avoided using information from RepBase.
Features
- Automated primer design
- Quality control through detailed settings
- Using well-known sources: Primer3, EnsEMBL, RepBase
- Creating your primers since 2003
- Website running as a service
- Perl code available for local execution
Audience
Science/Research, Advanced End Users
User interface
Web-based, Command-line
Programming Language
Perl
Categories
This is an application that can also be fetched from https://sourceforge.net/projects/autoprime/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.