This is the Linux app named Calis-p whose latest release can be downloaded as Calisp-2.1.zip. It can be run online in the free hosting provider OnWorks for workstations.
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Calis-p
DESCRIPTION
Calis-p (The CALgary approach to ISotopes in proteomics) is a java application to estimate isotopic composition (e.g. delta13C or delta15N) of individual species in a microbial community from a proteomic dataset. Calis-p 2.0 handles both natural isotope abundances and data from labelling experiments such as stable isotope probing (SIP). It requires a mzIdent (or target spectrum match) and mzML files as the input and requires about 1 min per mzML file with 10 threads and needs <10 Gb of RAM. It has been tested with data from various nano liquid chromatography/Orbitrap platforms. For successful SIP, it is extremely sensitive, but requires the 13C fraction to remain below 10%.
Features
- delta13C is estimated from a standard metaproteomic dataset
- Efficently and reliably obtain delta13C for many species in a microbiome samples.
Categories
This is an application that can also be fetched from https://sourceforge.net/projects/calis-p/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.
