This is the Linux app named Digital Expression on the Web whose latest release can be downloaded as dew_RC2.tar.bz2. It can be run online in the free hosting provider OnWorks for workstations.
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Digital Expression on the Web
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DESCRIPTION
DEW is a platform that allows users to explore RNA-Seq data. A web-based Graphical User Interface is included.
The analysis proceeds as such: gapped alignments are performed and corrected for length, PCR and fragment bias so that a Fragment Per (effective) Kilobase per Million of reads (FPKM) is estimated as well as the simpler Reads Per Kb per Million of reads (RPKM). When provided with multiple isoforms and in the ‘contextual’ mode, corrections include a expectation maximization algorithm estimates effective expression profiles and a corrected alignment is produced. For each gene the user provides, DEW computes coverage descriptive statistics (RPKM, FKMP and total, mean and median corrected counts), expression profiles (normalized as R/FPKM and trimmed mean of fold change). DEW allows users to explore the data by providing interactive graphs.
Audience
Science/Research
User interface
Web-based
Programming Language
Perl, JavaScript, S/R
Database Environment
SQLite
Categories
This is an application that can also be fetched from https://sourceforge.net/projects/dew/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.