This is the Linux app named easy-dhpsf to run in Linux online whose latest release can be downloaded as easy-dhpsf_v2_1.zip. It can be run online in the free hosting provider OnWorks for workstations.
Download and run online this app named easy-dhpsf to run in Linux online with OnWorks for free.
Follow these instructions in order to run this app:
- 1. Downloaded this application in your PC.
- 2. Enter in our file manager https://www.onworks.net/myfiles.php?username=XXXXX with the username that you want.
- 3. Upload this application in such filemanager.
- 4. Start the OnWorks Linux online or Windows online emulator or MACOS online emulator from this website.
- 5. From the OnWorks Linux OS you have just started, goto our file manager https://www.onworks.net/myfiles.php?username=XXXXX with the username that you want.
- 6. Download the application, install it and run it.
easy-dhpsf to run in Linux online
DESCRIPTION:
This package of MATLAB routines with accompanying GUI allows scientific users to measure the 3D location of single molecules when using the double-helix point spread function (DH-PSF) widefield microscope. Version 2 also includes code that facilitates combining localization data from two spectral channels using a locally-weighted quadratic 3D registration functionFeatures
- Double-Gaussian estimator is calibrated via an axial scan of bright immobile fluorescent emitters (e.g. beads)
- DH localization is performed through template matching and subsequent double Gaussian fitting
- Tiff stacks of SM images are analyzed using template matching followed by double-Gaussian fitting to extract estimates of the molecule positions
- Two spectral channels are registered by generating a locally-weighted quadratic mapping function from control point pairs
Audience
Science/Research
Programming Language
MATLAB
This is an application that can also be fetched from https://sourceforge.net/projects/easy-dhpsf/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.