This is the Windows app named PepDistiller whose latest release can be downloaded as PD_1_28_release_20140702.7z. It can be run online in the free hosting provider OnWorks for workstations.
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PepDistiller
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DESCRIPTION
In this study, we presented a quality control tool named PepDistiller to facilitate the validation of MASCOT search results. By including the number of tryptic termini, and integrating a refined false discovery rate (FDR) calculation method, we demonstrated the improved sensitivity of peptide identifications obtained from semitryptic search results. Based on the analysis of a complex data set, approximately 7% more peptide identifications were obtained using PepDistiller than using MASCOT Percolator. Moreover, the refined method generated lower FDR estimations than the percentage of incorrect target (PIT) fixed method applied in Percolator. Using a standard data set, we further demonstrated the increased accuracy of the refined FDR estimations relative to the PIT-fixed FDR estimations. PepDistiller is fast and convenient to use, and is freely available for academic access. The software can also be downloaded from http://www.bprc.ac.cn/pepdistiller.
Features
- Quality Control
- Proteomics
- Peptide Identification
Categories
This is an application that can also be fetched from https://sourceforge.net/projects/pepdistiller/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.
